Article | . 2017 Vol. 17, Issue. 3
Effect of Cimicifuga rhizoma extract on the odontoblastic differentiation of MDPC-23 cells



Department of Oral Anatomy, School of Dentistry, Pusan National University1
Department of Dental Hygiene, Kyungnam College of Information & Technology2




2017.. 441:8


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The purpose of this study was to examine the cell proliferation and expression of alkaline phosphatase (ALP) during the differentiation of murine odontoblast-like cells (MDPC-23) by Cimicifuga rhizoma extract. Cimicifuga rhizoma extract was prepared using 70% ethanol. Then, the cells were treated with 25, 50, 100, 150, and 200 µg of Cimicifuga rhizoma extract. Methods: We determined the Cimicifuga rhizoma effects of MDPC-23 using WST-1 (water soluble tetrazolium salt-1) assay, ALP activity assay and histochemical staining. Results: 25-200 µg of Cimicifuga rhizoma extract did not inhibit the growth of MDPC-23 cells; 100±0, 100±3.29, 99±4.86, 98±3.80, 98±1.73, 99±5.05% (p<0.794). 50 µg of Cimicifuga rhizoma extract stimulated ALP activity on MDPC-23; 5.1±0.20 units/µℓ (p<0.001). Conclusions: It was proven that Cimicifuga rhizoma promoted differentiation of MDPC- 23 cells.



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