Department of Preventive and Social Dentistry, School of Dentistry, Kyung Hee University1
Department of Dental Hygiene, Hanseo University2
Objectives: This study aims to evaluate carbonated drinks induced dental caries with qualitative analysis and to compare with oral organic acids including lactate, acetate, propionate, formate, butyrate, pyruvate and valerate which cause caries when taking either 10% sucrose drinks or carbonated drinks. Methods: Saliva was collected from six study subjects before and after (start, 5, 10, 30 minutes) taking water intake upon (A) 10% sucrose intake, (B) 10% sucrose intake, and (C) carbonated drink intake, then they were centrifuged at 1,200 rpm followed by removing bacteria and enzymes with syringe filtering, performing a qualitative analysis with HPLC conductivity detection (GP50 gradient pump, ED 50 detector) after saliva pre-treatment under isocratic 100 mM NaOH mobile phase. Results: Higher risk of dental caries was evaluated in order of C>B>A, with the results of total oral organic acids’ concentration, lactates of organic acids and organic acids produced after 5 minutes from the 3 types of drinks intake. Conclusions: Carbonated beverages were estimated to develop higher dental caries induction than beverages containing 10% sucrose because of the high organic acid concentration in the mouth after its intake.
1. Hwang EJ, Yoo JH, Shin JY, Bae MJ, Jo SI. Association of sugar-sweetened beverages consumption and hypertension in Korean adults: Korean National Health and Nutrition Examination Survey 2012-2013. Korean J Fam Pract 2016;6:446-5. https://doi.org/10.21215/ kjfp.2016.6.5.446
2. WHO technical staff. Reducing consumption of sugar-sweetened beverages to reduce the risk of unhealthy weight gain in adults. e-Library of Evidence for Nutrition Actions (eLENA). September 2014. [Cited 2017 Feb 20]. https://www.who.int/elena/titles/bbc/ssbs_adult_weight/en/
3. Srinath Reddy K, Katan MB. Diet, nutrition and the prevention of hypertension and cardio-vascular diseases. Public Health Nutr 2004;7:167-86. https://doi.org/10.1079/phn2003587
4. Steyn NP, Mann J, Bennett PH, Temple N, Zimmet P, Tuomilehto J et al. Diet, nutrition and the prevention of type 2 diabetes. Public Health Nutr 2004;7:147-65. https://doi.org/10.1079/ phn2003586
5. Adegboye AR, Twetman S, Christensen LB, Heitmann BL. Intake of dairy calcium and tooth loss among adult Danish men and women. Nutrition 2012;28:779-84. https://doi.org/10.1016/ j.nut.2011.11.011
6. Somborac M. Improving nutrition for better oral health. J Can Dent Assoc 2010;76:a131.
7. Lussi A, Jaeggi T, Zero D. The role of diet in the aetiology of dental erosion. Caries Res 2004; 38:34-44. https://doi.org/10.1159/000074360
8. Danielsson Niemi L, Hernell O, Johansson I. Human milk compounds inhibiting adhesion of mutans streptococci to host ligand-coated hydroxyapatite in vitro. Caries Res 2009;43:171-8. https://doi.org/10.1159/000213888
9. Kashket S, DePaola DP. Cheese consumption and the development and progression of dental caries. Nutr Rev 2002;60:97-103. https://doi.org/10.1301/00296640260085822
10. Imfeld T. Dental erosion. Definition, classification and links. Eur J Oral Sci 1996;104:151-5. https://doi.org/10.1111/j.1600-0722.1996.tb00063.x
11. Cavalcanti AL, Costa Oliveira M, Florentino VG, dos Santos JA, Vieira FF, Cavalcanti CL. Short communication: in vitro assessment of erosive potential of energy drinks. Eur Arch Paediatr Dent 2010;11:253-5. https://doi.org/10.1007/bf03262757
12. Pinto SC, Bandeca MC, Silva CN, Cavassim R, Borges AH, Sampaio JE. Erosive potential of energy drinks on the dentine surface. BMC Res Notes 2013;6:67. https://doi.org/10.1186/1756- 0500-6-67
13. Kang JO, Jang JH. Investigation of cariogenic effects of commercial sports drinks by HPLC analysis. Int J Clin Prev Dent 2013;9:231-8.
14. Yoo SM, Park YD, Ahn GS. Analysis of dental cariogenic carboxylic acids in 10 kinds of energy drinks. Int J Clin Prev Dent 2013;9:53-8.
15. Yoshida Y, Van Meerbeek B, Nakayama Y, Yoshioka M, Snauwaert J, Abe Y, et al. Adhesion to and decalcification of hydroxyapatite by carboxylic acids. J Dent Res 2001;80:1565-9. https://doi.org/10.1177/00220345010800061701
16. Hannig C, Hamkens A, Becker K, Attin R, Attin T. Erosive effects of different acids on bovine enamel: release of calcium and phosphate in vitro. Arch Oral Biol 2005;50:541-52. https:// doi.org/10.1016/j.archoralbio.2004.11.002
17. Keyes PH. Present and future measures for dental caries control. J Am Dent Assoc 1969;79: 1395-404. https://doi.org/10.14219/jada.archive.1969.0037
18. Lussi A, von Salis-Marincek M, Ganss C, Hellwig E, Cheaib Z, Jaeggi T. Clinical study monitoring the pH on tooth surfaces in patients with and without erosion. Caries Res 2012; 46:507-12. https://doi.org/10.1159/000339783
19. Owens BM, Kitchens M. The erosive potential of soft drinks on enamel surface substrate: an in vitro scanning electron microscopy investigation. J Contemp Dent Pract 2007;8:11-20.
20. Fanguy JC, Henry CS. The analysis of uric acid in urine using microchip capillary electro-phoresis with electrochemical detection. Electrophoresis 2002;23:767-73. https://doi.org/10.1002/ 1522-2683(200203)23:5<767::aid-elps767>3.3.co;2-#
21. Guo WP, Lau KM, Fung YS. Microfluidic chip-capillary electrophoresis for two orders extension of adjustable upper working range for profiling of inorganic and organic anions in urine. Electrophoresis 2010;31:3044-52. https://doi.org/10.1002/elps.201000297
22. Buescher JM, Moco S, Sauer U, Zamboni N. Ultrahigh performance liquid chromatography-tandem mass spectrometry method for fast and robust quantification of anionic and aromatic metabolites. Anal Chem 2010;82(11):4403-12. https://doi.org/10.1021/ac100101d
23. Birkler RI, Støttrup NB, Hermannson S, Nielsen TT, Gregersen N, Bøtker HE, et al. A UPLC-MS/MS application for profiling of intermediary energy metabolites in microdialysis samples--a method for high-throughput. J Pharm Biomed Anal 2010;53:983-90. https://doi.org/ 10.1016/j.jpba.2010.06.005
24. Paik MJ, Cho EY, Kim H, Kim KR, Choi S, Ahn YH, et al. Simultaneous clinical monitoring of lactic acid, pyruvic acid and ketone bodies in plasma as methoxime/tert-butyldimethylsilyl derivatives by gas chromatography-mass spectrometry in selected ion monitoring mode. Biomed Chromatogr 2008;22:450-3. https://doi.org/10.1002/bmc.966
25. Park YD, Jang JH, Oh YJ, Kwon HJ. Analyses of organic acids and inorganic anions and their relationship in human saliva before and after glucose intake. Arch Oral Biol 2014;59:1-11. https://doi.org/10.1016 /j.archoralbio. 2013.10.006
26. Takahashi N, Yamada T. Glucose and lactate metabolism by actinomyces naeslundii. Crit Rev Oral Biol Med 1999;10:487-503. https://doi.org/10.1177/10454411990100040501
27. Sommer P, Klein JP, Scholler M, Frank RM. Lactate dehydrogenase from streptococcus mutans: purification, characterization, and crossed antigenicity with lactate dehydrogenases from lactobacillus casei, actinomyces viscosus, and streptococcus sanguis. Infect Immun 1985;47: 489-95.
28. Gent-Ruijters ML, Meijere FA, Vries W, Stouthamer AH. Lactate metabolism in propionibac-terium pentosaceum growing with nitrate or oxygen as hydrogen acceptor. Antonie Van Leeuwenhoek 1976;42:217-28. https://doi.org/10.1007/bf00394118
29. Schweiger G, Buckel W. On the dehydration of (R)-lactate in the fermentation of alanine to propionate by clostridium propionocum. FEBS Lett 1984;171:79-84. https://doi.org/10.1016/ 0014-5793(84)80463-9
30. Oude Elferink SJ, Krooneman J, Gottschal JC, Spoelstra SF, Faber F, Driehuis F. Anaerobic conversion of lactic acid to acetic acid and 1,2-propanediol by Lactobacillus buchneri. Appl Environ Microbiol 2001;67:125-32. https://doi.org/10.1128/aem.67.1.125-132.2001
31. Zeng AP, Biebl H, Schlieker H, Deckwer WD. Pathway analysis of glycerol fermentation by Klebsiellu pneumoniae: Regulation of reducing equivalent balance and product formation. Enzyme Microb Technol 1993;15:770-9. https://doi.org/10.1016/0141-0229(93)90008-p
32. Hoshino E, Sato M. Production and degradation of formate by Veilllonella dispar ATCC 17745. J Dent Res 1986;65:903-5. https://doi.org/10.1177/00220345860650060801
33. Cai G, Jin B, Saint C, Monis P. Genetic manipulation of butyrate formation pathways in Clostridium bytyricum. J Biotechnol 2011;155:269-74. https://doi.org/10.1016/j.jbiotec.2011.07.004
34. Kenealy WR, Waselefsky DM. Studies on the substrate range of Clostridium kluyveri; the use of propanol and succinate. Arch Microbiol 1985; 141:187-94. https://doi.org/10.1007/bf00408056
35. Stephan RM. Changes in the hydrogen-ion concentration on tooth surfaces and in carious lesions. J Amer Dent Ass 1940;27:718-23. https://doi.org/10.14219/jada.archive.1940.0178
36. Margolis HC, Moreno EC. Kinetics of hydroxyapatite dissolution in acetic, lactic, and phosphoric acid solutions. Calcif Tissue Int 1992;50:137-43. https://doi.org/10.1007/bf00298791
37. Beyer M, Reichert J, Bossert J, Sigusch BW, Watts DC, Jandt KD. Acids with an equivalent taste lead to different erosion of human dental enamel. Dent Mater 2011;27:1017-23. https:// doi.org/10.1016/j.dental.2011.07.001
38. Yoo SM, Ahn GS. Correlation of oral microorganism and carboxylic acid in oral cavity. Int J Clin Prev Dent 2015;11(3):165-70. https://doi.org/10.15236/ijcpd.2015.11.3.16565
39. Jeon YJ, Choi JS, Han SJ. The effect of dry mouth improvement by oral exercise program in elderly people. J Korean Soc Dent Hyg 2012;12:293-305. https://doi.org/10.13065/jksdh. 2012.12.2.293
40. Kim SH. The effect of plaque control (tooth brushing instruction) for oral health improvement on periodontitis patients. J Korean Soc Dent Hyg 2011;11:293-301.